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biotinylated horse antimouse igg secondary antibody  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated horse antimouse igg secondary antibody
    Biotinylated Horse Antimouse Igg Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+horse+antimouse+igg+secondary+antibody/R%2ET%2EU%2E+Biotinylated+Horse+Anti-Mouse+IgG+Antibody+(Ready-to-Use)/pm41490873-233-20-29
    Average 93 stars, based on 28 article reviews
    biotinylated horse antimouse igg secondary antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Voluntary exercise induces adult hippocampal neurogenesis and BDNF expression in a rodent model of fetal alcohol spectrum disorders.
    Article Snippet: Alcohol consumption during pregnancy can result in a myriad of health problems in the affected offspring ranging from growth deficiencies to central nervous system impairments that result in cognitive deficits.. Adult hippocampal neurogenesis is thought to play a role in cognition (i.e. learning and memory) and can be modulated by extrinsic factors such as alcohol consumption and physical exercise.. We examined the impact of voluntary physical exercise on adult hippocampal neurogenesis in a rat model of fetal alcohol spectrum disorders (FASD).

    Article Title: Uterine Gα q/11 signaling, in a progesterone‐dependent manner, critically regulates the acquisition of uterine receptivity in the female mouse
    Article Snippet: Sections were then dehydrated in ethanol, cleared in xylene, overlaid with Permount mounting medium (Thermo Fisher Scientific), and sealed with a coverslip. .. Detection of PRs and phosphorylated PRs (phospho-PRs): Deparaffinized and rehydrated uterine sections were blocked with normal blocking serum (horse) and then incubated with mouse monoclonal anti-PR IgG (1:200 dilution, AB2764; Abcam, Cambridge,USAor rabbitpolyclonalphospho-PR(recognizesSer 294 on the human PR and homologous site on themouse PR) IgG (1:100 dilution, 13736; Cell Signaling, BostonMA, USA), or rabbit polyclonal phospho-PR (recognizes Ser 345 on the humanPRand homologous site on themousePR) IgG (1:100dilution, 12783;Cell SignalingTechnology)overnight at 4°C.Tissues incubated inantiPR IgG were then incubated with the biotinylated horse antimouse IgG secondary antibody (1:200 dilution, PK-1602; Vector Laboratories, Burlingame, CA,USA) for 1 h at room temperature. .. Phospho-PR IgGs were detected with the ImmunoCruz rabbit ABC Staining System without any adaptations (sc-2018; Santa Cruz Biotechnology, Dallas, TX, USA).

    Article Title: Serum HtrA1 is differentially regulated between early-onset and late-onset preeclampsia.
    Article Snippet: .. Following this, plates were incubated with mouse monoclonal anti-HtrA1 antibody (0.5 mg/ml in PBST) for 1 h, a biotinylated horse antimouse IgG secondary antibody (150 mg/ml in PBST; Vector Laboratories, Burlingame, CA, USA) for 40 min, and streptavidin-HRP (Dako, Carpinteria, CA, USA, diluted 1:1000 in PBST) for 30 min. .. Between each of these incubations, plates werewashed three times with PBST with 1 min soaking at each wash. Plates were then incubated with 50 ml/well of 1-Step Ultra TMB (Thermo Fisher Scientific, Rockford, IL, USA) in the dark for 10 min. To stop the reaction, 25 ml of 1M H2SO4 were added to each well, and absorbance at 450 nmwas determined (Wallac EnVision Multilabel Plate reader, Perkin Elmer, Waltham, MA, USA).

    Article Title: Needle biopsy-derived myofascial tissue samples are sufficient for quantification of myofibroblast density.
    Article Snippet: Introduction: Quantification of myofibroblasts is a promising method for assessing tissue properties in the field of fascia research.. This is commonly performed by immunohistochemistry for -smooth muscle actin.. However, usually larger tissue samples sizes are required for quantification.

    Article Title: Chemogenetic activation of hippocampal area CA2 promotes acute and chronic seizures in a mouse model of epilepsy.
    Article Snippet: .. On the following day, sections were washed in TB (3 × 5 min) and then incubated for 60 min in biotinylated horse antimouse IgG secondary antibody (1:500, Cat# BP-2000, Vector) diluted in a solution of 0.25 % Triton-X 100, and 1 % bovine serum albumin in TB. .. The sections were then washed in TB (3 × 5 min) and incubated in avidin-biotin complex for 2 h (1:1000; Cat# PK-6100, Vector Laboratories).

    Article Title: The study of myogenin expression in denervated human skeletal muscles.
    Article Snippet: Skeletal muscle denervation eventually causes atrophy as a result of interrupted nerve conduction and the lack of nutritional factors.. Myogenin is a myogenic regulatory factor that plays a key role in myoblast differentiation.. Changes in myogenin expression in denervated rat skeletal muscle have been demonstrated, but myogenin expression in denervated human skeletal muscle has not been reported.

    Article Title: Chemogenetic activation of hippocampal area CA2 promotes acute and chronic seizures in a mouse model of epilepsy
    Article Snippet: .. On the following day, sections were washed in TB (3 × 5 min) and then incubated for 60 min in biotinylated horse antimouse IgG secondary antibody (1:500, Cat# BP-2000, Vector) diluted in a solution of 0.25 % Triton-X 100, and 1 % bovine serum albumin in TB. .. The sections were then washed in TB (3 × 5 min) and incubated in avidin-biotin complex for 2 h (1:1000; Cat# PK-6100, Vector Laboratories).

    Avidin-Biotin Assay:

    Article Title: Voluntary exercise induces adult hippocampal neurogenesis and BDNF expression in a rodent model of fetal alcohol spectrum disorders.
    Article Snippet: Alcohol consumption during pregnancy can result in a myriad of health problems in the affected offspring ranging from growth deficiencies to central nervous system impairments that result in cognitive deficits.. Adult hippocampal neurogenesis is thought to play a role in cognition (i.e. learning and memory) and can be modulated by extrinsic factors such as alcohol consumption and physical exercise.. We examined the impact of voluntary physical exercise on adult hippocampal neurogenesis in a rat model of fetal alcohol spectrum disorders (FASD).

    Article Title: The study of myogenin expression in denervated human skeletal muscles.
    Article Snippet: Skeletal muscle denervation eventually causes atrophy as a result of interrupted nerve conduction and the lack of nutritional factors.. Myogenin is a myogenic regulatory factor that plays a key role in myoblast differentiation.. Changes in myogenin expression in denervated rat skeletal muscle have been demonstrated, but myogenin expression in denervated human skeletal muscle has not been reported.

    Blocking Assay:

    Article Title: Uterine Gα q/11 signaling, in a progesterone‐dependent manner, critically regulates the acquisition of uterine receptivity in the female mouse
    Article Snippet: Sections were then dehydrated in ethanol, cleared in xylene, overlaid with Permount mounting medium (Thermo Fisher Scientific), and sealed with a coverslip. .. Detection of PRs and phosphorylated PRs (phospho-PRs): Deparaffinized and rehydrated uterine sections were blocked with normal blocking serum (horse) and then incubated with mouse monoclonal anti-PR IgG (1:200 dilution, AB2764; Abcam, Cambridge,USAor rabbitpolyclonalphospho-PR(recognizesSer 294 on the human PR and homologous site on themouse PR) IgG (1:100 dilution, 13736; Cell Signaling, BostonMA, USA), or rabbit polyclonal phospho-PR (recognizes Ser 345 on the humanPRand homologous site on themousePR) IgG (1:100dilution, 12783;Cell SignalingTechnology)overnight at 4°C.Tissues incubated inantiPR IgG were then incubated with the biotinylated horse antimouse IgG secondary antibody (1:200 dilution, PK-1602; Vector Laboratories, Burlingame, CA,USA) for 1 h at room temperature. .. Phospho-PR IgGs were detected with the ImmunoCruz rabbit ABC Staining System without any adaptations (sc-2018; Santa Cruz Biotechnology, Dallas, TX, USA).



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